Molecular Minutes

RNA Mango: Track your RNA in Action!

Posted by Applied Biological Materials (abm) on January 28, 2020
Track your RNA in action with the novel RNA Mango fluorescent imaging system
 
 
Unrau_Photo
 
Technology for studying the diverse cellular roles of RNA has lagged behind the tools for studying DNA and proteins, but innovative researchers are working to change that! One such researcher is Dr. Peter Unrau of Simon Fraser University. He and his team have created RNA Mango, a novel technology with a number of useful applications. 
 
Key Features: 
  • Non-toxic dye + aptamer system
  • High affinity, sensitive imaging of even low concentration of RNA
  • Small, easy-to-insert RNA aptamer tags
  • Works in vitro and vivo

 

Applications: 
  • Pulldown RNA and RNPs in their native state
  • Measure gene transcription in real time
  • Track RNA/RNP abundance and distribution
 
How does RNA Mango Fluorescent Imaging System work?
RNA-MANGO
 
Now Available:
    • TO1-3PEG-Biotin Fluorophore (G955)
    • TO1-3PEG-Desthiobiotin Fluorophore (G956)
    • TO3-3PEG-Biotin Flurophore (G959)

 

Visit our website here to learn more about RNA Mango and to view our products!

 

Watch this video to see RNA Mango in Action!
 
RNA Mango

 

Transcription reaction were carried out in 300 µL volumes using T7 RNA polymerase (400 U, 50U/µL, applied biological materials), 0.5 µM TO1-3PEG-Biotin (applied biological materials), in 8 mM GTP, 5 mM CTP and ATP, 2 mM UTP, 40 mM TRIS buffer pH 7.9, 2.5 mM spermidine, 26 mM MgCl2, 20 mM KCl, Pyrophosphatase (0.5 U, 0.1 U/µL, ThermoFisher Scientific), and 0.01% Triton X-100. To each sample, either water (Negative), 0.33 µM DNA template (Mango Transcription), or 500 nM final Mango III A10U RNA (Positive) was added. Samples were visualized in a blue light box, movie is played back at 30X speed.

Topics: RNA Mango

Molecular Minutes

Educational resources for life scientists and interviews with scientists/science communicators in the field.

For more in-depth articles, check out our knowledge base, which covers topics such as CRISPR, Next Generation Sequencing, PCR, Cell Culture, and more.

Blog managed by Applied Biological Materials (abm). 

Subscribe to Email Updates

Recent Posts