Molecular Minutes

Q&A: How to Design, Run, and Optimize Your Viral Gene Delivery Experiment Webinar

Posted by Applied Biological Materials (abm) on June 25, 2019

Here is the full list of answers to questions we've received during the abm Viral Vectors - How to Design, Run, and Optimize Your Gene Delivery Experiment webinar:

  1. What is titer?
  2. How do I decide what virus titer to use? Is there a guideline for in vitro work? In vivo work?
  3. Can you please tell me what is DNA integration and why does it matter to decide which viral vector to use?
  4. How safe are the recombinant viruses? If I get them, do I need special facility to handle it?
  5. Do you have to ship viruses on dry ice or is blue ice ok?

1. What is titer?

Titer refers to the amount of virus in a solution, and it is a way of expressing concentration. For example, if we say a lentivirus has a titer of 1x106 IU/ml, this means that there is 1 million viral particles per millilitre, where IU stands for Infectious Units.


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Topics: Viral Vectors, Lentivirus, AAV

Meet AAV: The small but determined virus

Posted by Applied Biological Materials (abm) on January 29, 2019

In the third addition to our Vectors and Viruses Spotlight Series, we'll introduce you to the Adeno-associated virus (AAV), a small but powerful viral delivery system. An up-and-coming hero of the gene therapy world, AAVs are being used to deliver gene therapies for a wide range of diseases - from heart failure to retinal dystrophy - accounting for almost 8% of all vectors used in gene therapy clinical trials. Learn the advantages of choosing to use AAV in your experiments.
 

 

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Topics: Viral Vectors, AAV

Meet your friendly neighborhood gene delivery crew!

Posted by Applied Biological Materials (abm) on January 10, 2019

Delivering genes into cells isn’t easy—especially when the gene is bulky and sticky or if the cell is stubborn and curmudgeonly. But, not to worry, our gene delivery crew is so charming that your cells will lay down the welcome mat!

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Topics: Viral Vectors, Lentivirus, Adenovirus, AAV, Retrovirus

Molecular Minutes

Educational resources for life scientists and interviews with scientists/science communicators in the field.

For more in-depth articles, check out our knowledge base, which covers topics such as CRISPR, Next Generation Sequencing, PCR, Cell Culture, and more.

Blog managed by Applied Biological Materials (abm). 

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