Molecular Minutes

Q&A: Using NGS for CRISPR Validation, Antibody Screening, & Metagenomics

Posted by Applied Biological Materials (abm) on October 25, 2019

Here is the full list of answers to questions we've received during "Using NGS for CRISPR Validation, Antibody Screening, & Metagenomics" Amplicon-Seq webinar:

    1. If I have already done sequencing, but need help with the analysis, can you help me?
    2. What is the largest size of amplicon that I can have sequenced?
    3. What is the typical turnaround time for Amplicon-Seq, and can you offer an expedited service if I am in a rush?
    4. Can I do Amplicon-Seq to look for off-target effects as part of CRISPR validation?
    5. If I’m interested in metagenomics sequencing, can I do Amplicon-Seq for targets other than 16S, like fungi or other eukaryotes?
    6. Do you guarantee a minimum number of reads for projects or samples?
    7. When discussing coverage, does 50X mean 50 reads?
    8. I'm interested in CRISPR validation and metabolomics. Can I use Amplicon-Seq to look at metabolites?
    9. Is Krona the software used for identify species for metagenomic sequencing? If not, how do we determine the specific microorganisms present in our samples?
    10. Can you help design gRNA's for my CRISPR experiment?
    11. What are some considerations for using the HiSeq 4000 platform?
    12. When discussing read length, what does 2x300 bp PE mean?
    13. If I have already done sequencing, but need help with the analysis, can you help me?

 

 

1. If I have already done sequencing, but need help with the analysis, can you help me?

Our in-house bioinformatics team can assist with nearly any type of analysis (in as little as 1 week!) based on your project needs. In the near future, we will be launching a dedicated Bioinformatics Service for researchers, but if you have a project where you need help with analysis, you can contact our NGS team at ngs@abmgood.com. One of our team members can then help you with analysis you are interested in completing!


 

2. What is the largest size of amplicon that I can have sequenced?

For most projects, we can sequence amplicons that are <600bp. If you have an amplicon that is larger than this, please let us know, and we may be able to work with you on a customized solution for your project.


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Topics: CRISPR, Next Generation Sequencing, NGS, Amplicon-Seq, Metagenomics

Molecular Minutes

Educational resources for life scientists and interviews with scientists/science communicators in the field.

For more in-depth articles, check out our knowledge base, which covers topics such as CRISPR, Next Generation Sequencing, PCR, Cell Culture, and more.

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