Molecular Minutes

A Guide to Lentivirus Production (Protocol, Tips, & more!)

Posted by Applied Biological Materials (abm) on May 1, 2020

Lentiviruses are a key tool in today’s field of biology as they provide a reliable way to achieve stable over-expression of a gene in your cells of interest. However, many scientists are confused about how to produce these genetic engineering marvels in their own labs.

In this blog post, we'll outline the steps for packaging lentiviruses, including:

  1. Considerations before you start
  2. Basic packaging protocol
  3. Infection test experiment
  4. Storing your lentiviruses
  5. Preventing contamination
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Topics: Tips and Tricks, Lentivirus, Cell Culture

Your Basic Guide to Cell Line Immortalization

Posted by Applied Biological Materials (abm) on February 12, 2020

 

You’ve probably heard the saying, “all good things come to an end” - especially when it comes to precious primary cell cultures that reach senescence after a few passages. Thanks to the development of immortalized cell lines, however, this is no longer true! In this blog post we’ll go over:

  1. What are immortalized cells
  2. Strategies for generating immortalized cells
  3. An overview of the immortalization workflow
  4. Cell line quality control considerations
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Topics: Cell Culture, Cell line

#ScientistSpotlight - Yanquan Zhang and Huitao Liu

Posted by Applied Biological Materials (abm) on October 21, 2019

Meet Yanquan Zhang and Huitao Liu - our Spotlight Scientists. Enter our abmXchange program to be featured and get free lab materials! 

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Topics: Cell Culture, Adenovirus, abmXchange, Plasmid, Cell line

5 Steps to a Contamination-free Cell Culture Experience

Posted by Applied Biological Materials (abm) on December 6, 2018

No cell biologist wants to hear the words "cell culture contamination". Unfortunately, it is more commonplace in labs than it should be.

While some contaminants are visible to the eye, others are often left undetected due to their lack of visible appearance. This can have serious consequences when it comes to integrity of your experimental results.

Follow the 5 best practices below, and you can minimize your risk of contamination in your cell cultures.

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Topics: Tips and Tricks, Cell Culture

Molecular Minutes

Educational resources for life scientists and interviews with scientists/science communicators in the field.

For more in-depth articles, check out our knowledge base, which covers topics such as CRISPR, Next Generation Sequencing, PCR, Cell Culture, and more.

Blog managed by Applied Biological Materials (abm). 

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