Molecular Minutes

A Guide to Lentivirus Production (Protocol, Tips, & more!)

Posted by Applied Biological Materials (abm) on May 1, 2020

Lentiviruses are a key tool in today’s field of biology as they provide a reliable way to achieve stable over-expression of a gene in your cells of interest. However, many scientists are confused about how to produce these genetic engineering marvels in their own labs.

In this blog post, we'll outline the steps for packaging lentiviruses, including:

  1. Considerations before you start
  2. Basic packaging protocol
  3. Infection test experiment
  4. Storing your lentiviruses
  5. Preventing contamination
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Topics: Tips and Tricks, Lentivirus, Cell Culture

Q&A: How to Design, Run, and Optimize Your Viral Gene Delivery Experiment Webinar

Posted by Applied Biological Materials (abm) on June 25, 2019

Here is the full list of answers to questions we've received during the abm Viral Vectors - How to Design, Run, and Optimize Your Gene Delivery Experiment webinar:

  1. What is titer?
  2. How do I decide what virus titer to use? Is there a guideline for in vitro work? In vivo work?
  3. Can you please tell me what is DNA integration and why does it matter to decide which viral vector to use?
  4. How safe are the recombinant viruses? If I get them, do I need special facility to handle it?
  5. Do you have to ship viruses on dry ice or is blue ice ok?

1. What is titer?

Titer refers to the amount of virus in a solution, and it is a way of expressing concentration. For example, if we say a lentivirus has a titer of 1x106 IU/ml, this means that there is 1 million viral particles per millilitre, where IU stands for Infectious Units.


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Topics: Viral Vectors, Lentivirus, AAV

Meet Lentivirus: The resident charmer of mammalian cells!

Posted by Applied Biological Materials (abm) on January 10, 2019

In the first addition to our Vectors and Viruses Spotlight Series, learn what makes lentivirus different from other viral delivery systems, and the advantages of choosing to use lentivirus in your experiments.

 

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Topics: Viral Vectors, Lentivirus

Meet your friendly neighborhood gene delivery crew!

Posted by Applied Biological Materials (abm) on January 10, 2019

Delivering genes into cells isn’t easy—especially when the gene is bulky and sticky or if the cell is stubborn and curmudgeonly. But, not to worry, our gene delivery crew is so charming that your cells will lay down the welcome mat!

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Topics: Viral Vectors, Lentivirus, Adenovirus, AAV, Retrovirus

4 Things to Consider When Making Lentiviral Constructs

Posted by Applied Biological Materials (abm) on October 3, 2018

Lentivirus is one of the most commonly used gene delivery systems, yet there are many things to consider before using it for your experiments.

Here are 4 things you should consider when making your lentiviral constructs:

  1. Is lentivirus the right gene delivery system for your experiment?
  2. Is your sequence within the size limit?
  3. Have you optimized your vector backbone design?
  4. Are you choosing the right packaging system?

Find out the answers to these questions below, plus some additional tips for your next experiment.

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Topics: Tips and Tricks, Viral Vectors, Lentivirus

Molecular Minutes

Educational resources for life scientists and interviews with scientists/science communicators in the field.

For more in-depth articles, check out our knowledge base, which covers topics such as CRISPR, Next Generation Sequencing, PCR, Cell Culture, and more.

Blog managed by Applied Biological Materials (abm). 

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