Molecular Minutes

A Guide to Lentivirus Production (Protocol, Tips, & more!)

Posted by Applied Biological Materials (abm) on May 1, 2020

Lentiviruses are a key tool in today’s field of biology as they provide a reliable way to achieve stable over-expression of a gene in your cells of interest. However, many scientists are confused about how to produce these genetic engineering marvels in their own labs.

In this blog post, we'll outline the steps for packaging lentiviruses, including:

  1. Considerations before you start
  2. Basic packaging protocol
  3. Infection test experiment
  4. Storing your lentiviruses
  5. Preventing contamination
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Topics: Tips and Tricks, Lentivirus, Cell Culture

5 Steps to a Contamination-free Cell Culture Experience

Posted by Applied Biological Materials (abm) on December 6, 2018

No cell biologist wants to hear the words "cell culture contamination". Unfortunately, it is more commonplace in labs than it should be.

While some contaminants are visible to the eye, others are often left undetected due to their lack of visible appearance. This can have serious consequences when it comes to integrity of your experimental results.

Follow the 5 best practices below, and you can minimize your risk of contamination in your cell cultures.

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Topics: Tips and Tricks, Cell Culture

4 Things to Consider When Making Lentiviral Constructs

Posted by Applied Biological Materials (abm) on October 3, 2018

Lentivirus is one of the most commonly used gene delivery systems, yet there are many things to consider before using it for your experiments.

Here are 4 things you should consider when making your lentiviral constructs:

  1. Is lentivirus the right gene delivery system for your experiment?
  2. Is your sequence within the size limit?
  3. Have you optimized your vector backbone design?
  4. Are you choosing the right packaging system?

Find out the answers to these questions below, plus some additional tips for your next experiment.

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Topics: Tips and Tricks, Viral Vectors, Lentivirus

13 Practical Tips for Using Lab Freezers

Posted by Applied Biological Materials (abm) on October 2, 2018

Did you know your freezer consumes a large fragment of your energy and resources?

Lab freezers are usually the highest energy consumers in most Biological labs. Aging freezers consume as much as 3% more energy every consecutive year which can add up to $1,500 USD annually in energy bills.

What can you do to reduce this energy drain? 

Here are some tips and tricks on how you can keep your energy bills low and your ULT Freezer in good shape. Protect your precious time-sensitive samples before it is too late.

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Topics: Tips and Tricks, Lab Equipment

Molecular Minutes

Educational resources for life scientists and interviews with scientists/science communicators in the field.

For more in-depth articles, check out our knowledge base, which covers topics such as CRISPR, Next Generation Sequencing, PCR, Cell Culture, and more.

Blog managed by Applied Biological Materials (abm). 

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