Molecular Minutes

What is Multiplicity of Infection (MOI)?

Posted by Applied Biological Materials (abm) on January 1, 2020

So you’ve packaged your DNA into a virus and you’re ready to infect your cells! But, how many viral particles are required for 100% infection? In this blog post and video, we’ll explain the concept of Multiplicity of Infection or MOI and take you through how to determine the best MOI for your experiment.

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Topics: Viral Vectors, Multiplicity of Infection

Q&A: How to Design, Run, and Optimize Your Viral Gene Delivery Experiment Webinar

Posted by Applied Biological Materials (abm) on June 25, 2019

Here is the full list of answers to questions we've received during the abm Viral Vectors - How to Design, Run, and Optimize Your Gene Delivery Experiment webinar:

  1. What is titer?
  2. How do I decide what virus titer to use? Is there a guideline for in vitro work? In vivo work?
  3. Can you please tell me what is DNA integration and why does it matter to decide which viral vector to use?
  4. How safe are the recombinant viruses? If I get them, do I need special facility to handle it?
  5. Do you have to ship viruses on dry ice or is blue ice ok?

1. What is titer?

Titer refers to the amount of virus in a solution, and it is a way of expressing concentration. For example, if we say a lentivirus has a titer of 1x106 IU/ml, this means that there is 1 million viral particles per millilitre, where IU stands for Infectious Units.


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Topics: Viral Vectors, Lentivirus, AAV

Herpes Simplex Virus - Smart enough to deliver large transgenes to neurons

Posted by Applied Biological Materials (abm) on May 17, 2019

Say hello to the Herpes Simplex Virus (HSV), a resourceful virus that will find a way to deliver large transgenes into a variety of cells! In particular, it enjoys spending time in brainy locales like neurons, but this most courteous guest won't impose itself, being a non-integrative virus. In this seventh addition to our Vectors and Viruses Spotlight Series, find out why you should consider using the Herpes Simplex Virus (HSV) system for your gene expression projects!

 

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Topics: Viral Vectors, HSV

Baculovirus - Your best choice for producing glycoproteins

Posted by Applied Biological Materials (abm) on May 3, 2019

If you're looking to express glycoproteins or membrane proteins, then baculovirus is the perfect virus to help you with your gene expression experiments. In the sixth addition to our Vectors and Viruses Spotlight Series, we'll introduce you to the baculovirus system, a viral system that can delivery any gene, no matter how large. Learn more about baculovirus and why you should consider using it in your experiment in the article below!
 

 

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Topics: Viral Vectors, Baculovirus

Meet ORF: The Simple & Humble Team Player!

Posted by Applied Biological Materials (abm) on March 18, 2019

Sometimes less is more! Return to simpler times for an inexpensive way to get your gene of interest. In the fifth addition to our Vectors and Viruses Spotlight Series, we'll introduce you to ORF, a team player that can help you clone your gene into other vectors. Learn the advantages of choosing to use ORFs in your experiments in the article below!

 

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Topics: Viral Vectors, ORF

Choose Retrovirus For A Dance Party That Never Ends

Posted by Applied Biological Materials (abm) on February 20, 2019

Disco balls, bell bottoms, and perms, oh my! The retrovirus knows how to throw down a dance party with dividing cells. In the fourth addition to our Vectors and Viruses Spotlight Series, we'll introduce you to the retrovirus system, a viral system that only infects dividing cells. Any genes the retrovirus delivers will stick around in the cell long after the party's over. Learn what are the advantages of choosing to use retrovirus in your experiments in the article below!
 

 

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Topics: Viral Vectors, Retrovirus

Meet AAV: The small but determined virus

Posted by Applied Biological Materials (abm) on January 29, 2019

In the third addition to our Vectors and Viruses Spotlight Series, we'll introduce you to the Adeno-associated virus (AAV), a small but powerful viral delivery system. An up-and-coming hero of the gene therapy world, AAVs are being used to deliver gene therapies for a wide range of diseases - from heart failure to retinal dystrophy - accounting for almost 8% of all vectors used in gene therapy clinical trials. Learn the advantages of choosing to use AAV in your experiments.
 

 

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Topics: Viral Vectors, AAV

Meet Adenovirus: The reliable & efficient virus!

Posted by Applied Biological Materials (abm) on January 16, 2019

In the second addition to our Vectors and Viruses Spotlight Series, learn what makes adenovirus different from other viral delivery systems, and the advantages of choosing to use adenovirus in your experiments.
 

 

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Topics: Viral Vectors, Adenovirus

Meet Lentivirus: The resident charmer of mammalian cells!

Posted by Applied Biological Materials (abm) on January 10, 2019

In the first addition to our Vectors and Viruses Spotlight Series, learn what makes lentivirus different from other viral delivery systems, and the advantages of choosing to use lentivirus in your experiments.

 

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Topics: Viral Vectors, Lentivirus

Meet your friendly neighborhood gene delivery crew!

Posted by Applied Biological Materials (abm) on January 10, 2019

Delivering genes into cells isn’t easy—especially when the gene is bulky and sticky or if the cell is stubborn and curmudgeonly. But, not to worry, our gene delivery crew is so charming that your cells will lay down the welcome mat!

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Topics: Viral Vectors, Lentivirus, Adenovirus, AAV, Retrovirus

Molecular Minutes

Educational resources for life scientists and interviews with scientists/science communicators in the field.

For more in-depth articles, check out our knowledge base, which covers topics such as CRISPR, Next Generation Sequencing, PCR, Cell Culture, and more.

Blog managed by Applied Biological Materials (abm). 

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