Molecular Minutes

Meet Adenovirus: His infectious personality gets him into almost any cell!

Posted by Applied Biological Materials (abm) on January 16, 2019

In the second addition to our Vectors and Viruses Spotlight Series, learn what makes adenovirus different from other viral delivery systems, and the advantages of choosing to use adenovirus in your experiments.
 Viral-Expression-Systems-Character-Profiles_Adenovirus_blog

 

Meet Adenovirus

"Hello, I’m Andrew. If you need efficiency, I’m your virus! I have an infectious personality that gets me into almost any cell. If I deliver a gene, it will be expressed at high levels for a while, though I don’t like to outstay my welcome." 

 

Overview

Adenoviruses are medium-sized viruses with a linear dsDNA genome that is ~36 kb in length. Of this 36 kb, 8 kb can be used for carrying an expression cassette. Adenoviral DNA does not integrate into its host cell’s DNA. The DNA exists in the nucleus of the host cell, but is not replicated when the cell undergoes cell division. As the cells replicate over time, the adenoviral DNA expression weakens and eventually will disappear. Therefore, adenovirus is not a good choice for experiments that require long term, stable expression.

However, due to its excellent transduction efficiency (almost 100%) and high level of gene expression, adenovirus has been a popular choice for gene and shRNA expression for a variety of applications. These include vaccine production, gene therapy, gene knockdown, and the production of membrane proteins and antibodies. Adenoviruses have been already used in several gene therapy trials, particularly those targeting cancer cells. The first gene therapy product ever licensed was an adenovirus carrying the p53 repressor gene, for the treatment of head and neck squamous cell carcinoma.

While infection with adenovirus offers several advantages, the cloning of these viruses can be difficult and time consuming. Traditional methods involve the cloning of the transgene into a shuttle vector, then transferring the transgene into the adenoviral vector. As well, the adenoviral vector must be linearized before it can be packaged.

 

Choose adenovirus as your viral delivery system if:

  • Your insert cassette is smaller than 8 kb.
  • You do not want your gene integrated into the host genome.
  • You need high levels of transient expression.
  • You require a high transduction efficiency.
  • You wish to infect with a high titer of virus.

 

About abm's adenoviruses

  • Applications: Overexpression, silencing, CRISPR, and more
  • Genes available: Over 40,000 human, mouse, and rat genes offered
  • Promoters available: CMV
  • Tags and Reporters available: No tag, His tag, HA tag, GFP Reporter

 

We offer pre-cloned and packaged adenoviruses for use expressing anything from genes to shRNAs or sgRNAs.

abm offers the following genome-wide adenovirus collections:

 

Other adenovirus products and services we offer include:

 

Browse Adenovirus Library

 

Don't know which viral vector to choose?

You know you want to express a gene, but when it comes time to choose an expression vector, your choices can seem overwhelming. Do you go viral or non-viral? Which virus should you use? Or maybe it's not a protein you need to express, but an RNA species?

Try using our Vector Selection Tool. Cut through the fog with this Vector Selection Tool. Simple, clear recommendations for every application.

 

Go to Viral Vector Selector

 

Subscribe and stay tuned to our Molecular Minutes blog posts to meet the other crew members of the abm vector and virus collection.

 

Topics: Viral Vectors, Adenovirus

Molecular Minutes

Educational resources for life scientists and interviews with scientists/science communicators in the field.

For more in-depth articles, check out our knowledge base, which covers topics such as CRISPR, Next Generation Sequencing, PCR, Cell Culture, and more.

Blog managed by Applied Biological Materials (abm). 

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