In the second addition to our Vectors and Viruses Spotlight Series, learn what makes adenovirus different from other viral delivery systems, and the advantages of choosing to use adenovirus in your experiments.
"Hello, I’m Andrew. If you need efficiency, I’m your virus! I have an infectious personality that gets me into almost any cell. If I deliver a gene, it will be expressed at high levels for a while, though I don’t like to outstay my welcome."
OverviewAdenoviruses are medium-sized viruses with a linear dsDNA genome that is ~36 kb in length. Of this 36 kb, 8 kb can be used for carrying an expression cassette. Adenoviral DNA does not integrate into its host cell’s DNA. The DNA exists in the nucleus of the host cell, but is not replicated when the cell undergoes cell division. As the cells replicate over time, the adenoviral DNA expression weakens and eventually will disappear. Therefore, adenovirus is not a good choice for experiments that require long term, stable expression.
However, due to its excellent transduction efficiency (almost 100%) and high level of gene expression, adenovirus has been a popular choice for gene and shRNA expression for a variety of applications. These include vaccine production, gene therapy, gene knockdown, and the production of membrane proteins and antibodies. Adenoviruses have been already used in several gene therapy trials, particularly those targeting cancer cells. The first gene therapy product ever licensed was an adenovirus carrying the p53 repressor gene, for the treatment of head and neck squamous cell carcinoma.
While infection with adenovirus offers several advantages, the cloning of these viruses can be difficult and time consuming. Traditional methods involve the cloning of the transgene into a shuttle vector, then transferring the transgene into the adenoviral vector. As well, the adenoviral vector must be linearized before it can be packaged.
Choose adenovirus as your viral delivery system if:
- Your insert cassette is smaller than 8 kb.
- You do not want your gene integrated into the host genome.
- You need high levels of transient expression.
- You require a high transduction efficiency.
- You wish to infect with a high titer of virus.
About abm's adenoviruses
- Applications: Overexpression, silencing, CRISPR, and more
- Genes available: Over 40,000 human, mouse, and rat genes offered
- Promoters available: CMV
- Tags and Reporters available: No tag, His tag, HA tag, GFP Reporter
We offer pre-cloned and packaged adenoviruses for use expressing anything from genes to shRNAs or sgRNAs.
abm offers the following genome-wide adenovirus collections:
- Gene expression adenovirus collection
- miRNA and miRNA inhibitor adenovirus collection
- CRISPR sgRNA adenovirus collection
Other adenovirus products and services we offer include:
- Custom recombinant adenovirus , including siRNA adenovirus
- Adenovirus cloning and packaging systems , including for siRNA expression
- Adenovirus purification kits
- Pre-made adenoviruses for Cas9 expression , iPSC generation , and cell immortalization
Don't know which viral vector to choose?
You know you want to express a gene, but when it comes time to choose an expression vector, your choices can seem overwhelming. Do you go viral or non-viral? Which virus should you use? Or maybe it's not a protein you need to express, but an RNA species?
Try using our Vector Selection Tool. Cut through the fog with this Vector Selection Tool. Simple, clear recommendations for every application.
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