Sign Up for the Free CRISPR Crash Course
CRISPR has revolutionized the field of biotechnology. Don’t be left behind! This course will walk you through the practical applications of CRISPR Cas9, from experimental design to knockout validation.
CRISPR Cas9 – from start to finish
|How to design the best sgRNAs – and test them!
|Which Cas9 Nuclease is right for your experiment
|How to deliver and express CRISPR components in your cells
|Methods of edit validation
Experimental Overview of CRISPR Cas9 for Gene Knockout
Understand the big picture. We’ll go over the general workflow for a gene knockout experiment then help you decide which Cas9 nuclease is right for you.
sgRNA Design and Validation
Learn how to design your sgRNA and validate it for maximum efficiency. Test your sgRNA design skills.
sgRNA and Cas9 Delivery
Receive advice on what delivery method to use for your sgRNA and Cas9, with detailed protocols on how to proceed with gene knockout in your cell line.
Screening and Validation of Knockout
Decide how to screen and validate your cell line. A variety of methods will be discussed in detail.