Sign Up for the Free CRISPR Crash Course

How does it work?

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After signing up, each week we’ll send you new material for planning your CRISPR experiment. This 4-week Crash Course will teach you everything you need for a successful CRISPR knockout experiment!

Why is it important?

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CRISPR has revolutionized the field of biotechnology. Don’t be left behind! This course will walk you through the practical applications of CRISPR Cas9, from experimental design to knockout validation.

Topics Overview

CRISPR Cas9 – from start to finish

CRISPR-Crash-Course-check-icon_v2-08 How to design the best sgRNAs – and test them!

CRISPR-Crash-Course-check-icon_v2-08 Which Cas9 Nuclease is right for your experiment

CRISPR-Crash-Course-check-icon_v2-08 How to deliver and express CRISPR components in your cells
 
CRISPR-Crash-Course-check-icon_v2-08 Methods of edit validation 

 

Course Overview

WEEK 1


Experimental Overview of CRISPR Cas9 for Gene Knockout

Understand the big picture. We’ll go over the general workflow for a gene knockout experiment then help you decide which Cas9 nuclease is right for you.

Week 1

WEEK 2


sgRNA Design and Validation
      

Learn how to design your sgRNA and validate it for maximum efficiency. Test your sgRNA design skills.                                      

Week 2

 

WEEK 3


sgRNA and Cas9 Delivery

Receive advice on what delivery method to use for your sgRNA and Cas9, with detailed protocols on how to proceed with gene knockout in your cell line.    

Week 3

WEEK 4


Screening and Validation of Knockout

Decide how to screen and validate your cell line. A variety of methods will be discussed in detail.                                                                            

Week 4

Sign Up For The Free Course Today!