Technology for studying the diverse cellular roles of RNA has lagged behind the tools for studying DNA and proteins, but innovative researchers are working to change that! One such researcher is Dr. Peter Unrau of Simon Fraser University. He and his team have created RNA Mango, a novel technology with a number of useful applications.

Key Features:

• Non-toxic dye + aptamer system
• High affinity, sensitive imaging of even low concentration of RNA
• Small, easy-to-insert RNA aptamer tags
• Works in vitro and in vivo

Applications:

• Pulldown RNA and RNPs in their native state
• Measure gene transcription in real time
• Track RNA/RNP abundance and distribution

Now Available:

• TO1-3PEG-Biotin Fluorophore (G955)
• TO1-3PEG-Desthiobiotin Fluorophore (G956)
• TO3-3PEG-Biotin Flurophore (G959)
• YO3-3PEG-Biotin Fluorophore (G957)

Visit our website here to learn more about RNA Mango and to view our products!

Transcription reaction were carried out in 300 µL volumes using T7 RNA polymerase (400 U, 50U/µL, applied biological materials), 0.5 µM TO1-3PEG-Biotin (applied biological materials), in 8 mM GTP, 5 mM CTP and ATP, 2 mM UTP, 40 mM TRIS buffer pH 7.9, 2.5 mM spermidine, 26 mM MgCl2, 20 mM KCl, Pyrophosphatase (0.5 U, 0.1 U/µL, ThermoFisher Scientific), and 0.01% Triton X-100. To each sample, either water (Negative), 0.33 µM DNA template (Mango Transcription), or 500 nM final Mango III A10U RNA (Positive) was added. Samples were visualized in a blue light box, movie is played back at 30X speed.