All Categories
Cell Culture Resources Banner
Cell Culture Resources

In biology, cell culture is the process in which cells are grown in controlled conditions outside of its native environment. This technique has enabled scientists to use cells, the fundamental unit of life, as an imperative model system for understanding the physiological processes in the human body.

One of the major contributions of cell culture is the development of the polio vaccine. Back in the early 1930s, researchers had to use live animals to grow poliovirus, but with cell culture, researchers were able to have greater control of virus production which eventually led to the creation of the vaccine. Fast forwarding to the 1970s, mammalian cell culture was used to create properly folded and glycosilated proteins, such as inteferons and antibodies, that were impossible to make in prokaryotic E. coli cells because E. coli cells lack post-translational modifications. Nowadays, cell culture is being used widely in laboratory settings, particularly in the field of vaccine research, cancer research, and protein therapeutics

This collection of free resources will help you learn the essentials of cell culture theory and practice, including the basics of asceptic technique and cell culturing environments, cell immortalization protocols, recognizing and handling cell culture contamination, and more.

Articles
Cell Culture Introduction

Introduction

This knowledge base explains the essentials of cell culture, including primary vs. immortalized cells, the cell cycle and immortalization process, the cell growth environment, basic cell culture techniques, and more.

Growth Factors & Cytokines

Growth Factors & Cytokines

This knowledge base introduces the basics of growth factors and cytokines, including signalling mechanisms, growth factor classifications, applications of growth factors and cytokines, useful terms, and more.

Your Basic Guide to Cell Immortalization

Your Basic Guide to Cell Immortalization

This article explains the basics of immortalized cells, strategies for how to generate immortalized cells, an overview of a typical immortalization workflow, cell line quality control considerations, and more.

Cell Culture Quality Control and Contamination

Cell Culture Quality Control and Contamination

In this article, we'll go over how to identify and handle cells with bacterial, fungal, or microbial contamination, mycoplasma contamination, cell line cross-contamination, and virus or pathogen contamination.

Cell Culture Viability and Cell Attachment

Cell Culture Viability and Cell Attachment

This article covers how to maintain cell culture viability, including cell culture growth behavior, cell morphology, identifying cell death, cell couting, components of cell culture media/vessels, and a troubleshooting guide




Videos

Cell Culture Tutorial - An Introduction

Growth factors and Cytokines Tutorial - An Introduction

Cell Immortalization: How to Immortalize Cells




Have a question or suggestion?

Fill in the form below to submit your questions or suggestions on what you'd like us to cover for future knowledge base and video topics.

Top