circular RNA (circRNA) is a novel type of single-stranded, non-coding RNA molecule widely expressed in eukaryotes. Unlike typical RNA molecules, these circRNAs possess no 5’ cap or 3’ end polyA tail and instead form a ring structure joined by a covalent bond. Historically circRNAs were thought to be rare byproducts of aberrant splicing events. However, recent findings in the past two decades have revealed that they are widely expressed and have functionally significant roles in eukaryotes. Currently, a growing number of publications have implicated aberrant expression of circRNAs to be associated with diseases such as cancer, and this will likely remain a key focus point for researchers.
This collection of free resources will help you learn the essentials of circRNA including the basics of structure, function, biogenesis, methods of expression, enrichment and detection.
Introduction
Learn the basics of circular RNA including structural characteristics, models for biogenesis and diverse functions within the cell.
Methods of circRNA Expression
This article provides an overview of various strategies that can be employed to express circRNA in vivo and in vitro.
Methods of circRNA Enrichment, Detection & Analysis
Learn strategies to enrich your sample for circRNAs allowing for enhanced detection using RNA-Seq and downstream analysis tools.
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