Polymerase Chain Reaction (PCR) is a technology indispensible to genetic and molecular biology. It's widely use in the world around us, including applications in DNA sequencing, DNA fingerprinting, forensics, detection of microorganisms, and diagnosis of hereditary disease.
PCR allows fast and inexpensive amplification of DNA fragments. Because it is able to generate large quantities of DNA from a small amount of nucleic acid, PCR is also referred to as molecular photocopying. Our free collection of PCR resources includes articles and videos explaining the basic principles as well as practical applications and design considerations for PCR experiments.
Introduction
This article introduces you to the basics of polymerase chain reaction (PCR), including how it works (initialization, denaturation, annealing extension/elongation, etc.), types of DNA templates and strategies, primer design, buffer components, additives/inhibitors, thermal cycling conditions, and more.
Variations of DNA Polymerase
In this article, you'll learn about the characteristics, structures, and mechanisms of action (including fidelity and processivity) for common DNA polymerases such as Taq DNA Polymerase. We'll introduce you to pfu, KOD, long-range, hot-start, Bst, and Bsu DNA polymerases and more.
Real-Time PCR/Quantitative PCR (qPCR)
This article introduces you to real-time pcr (RT-PCR) and quantitative pcr (qPCR), focusing on TaqMan and SYBR Green qPCR, the advantages and disadvantages of each system, and their applications in research.
Reverse Transcription
This article covers the basics of reverse transcription or RT-PCR, including the mechanism, types of reverse transcriptases and primers, end-point PCR, real time PCR (qPCR), one-step and two-step RT-PCR, and experimental set-up notes and sample protocols.
PCR: Variations to the System
Polymerase chain reaction (PCR) is a versatile method. This article takes you through the many variations to the system, including multiplex PCR, nested PCR, asymmetric PCR, assembly PCR, touch down PCR, digital PCR, suicide PCR, VNTR PCR, PCR for site-directed mutagenesis, cold PCR and isothermal PCR.
Tips for successful PCR and Troubleshooting PCR/qPCR
A comprehensive guide on how to set-up a successful PCR, including avoiding contamination, designing primers, selecting appropriate reagents, and optimizing cycling conditions. Includes a troubleshooting guide for commonly observed problems, their potential causes, and recomended solutions.
PCR (Polymerase Chain Reaction) Tutorial - An Introduction
Polymerase Chain Reaction (PCR) - DNA Polymerase
Polymerase Chain Reaction (PCR) - Quantitative PCR (qPCR)
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