Next Generation Sequencing (NGS) is a powerful platform that has enabled the sequencing of thousands to millions of DNA molecules simultaneously. This powerful tool is revolutionizing fields such as personalized medicine, genetic diseases, and clinical diagnostics by offering a high throughput option with the capability to sequence multiple individuals at the same time.
Our collection of free resources includes an introduction into the theory, comparisons between different NGS technologies, experimental design (coverage, library preparation, and quality control), data analysis (raw data, software and tools), whole genome sequencing, and more.
Introduction
This knowledge base covers the basics of Sanger Sequencing and Next Generation Sequencing (NGS) and how these platforms work. You'll learn about the similarities and differences between different NGS technologies (in terms of sample prep, machines, and data output) as well as useful terms.
Experimental Design
This article explains how to design your own next generation sequencing (NGS) project and includes, an explanation of key terms (e.g. read, coverage, depth, etc.), considerations for WGS, Exome-Seq, RNA-Seq, and Methyl-Seq library preparation, quality control, and downstream applications.
Data Analysis
This knowledge base covers the basics of NGS Data Analysis, including how the raw sequencing data looks like, how to process the data for basic downstream analysis, and tools/software you can use.
Whole Genome Sequencing (WGS)
Learn the basics of Whole Genome Sequencing (WGS), including it's applications in de novo genome assembly, pathogen tracking, molecular evolution, and more.
Next Generation Sequencing (NGS) - An Introduction
Next Generation Sequencing (NGS) - Sample Preparation
Next Generation Sequencing (NGS) - Coverage & Sample Quality Control
Next Generation Sequencing (NGS) - Coverage & Sample Quality Control
Next Generation Sequencing (NGS) - Applications of Whole Genome Sequencing
Webinar: The Beginner's Guide to RNA-Seq
In this free 30 min webinar, you'll get a brief introduction to Next Generation Sequencing, learn important things to consider when designing your RNA-Seq experiment, understand the overall RNA-Seq workflow, anaysis, and interpretation, as well as go through a simple case study to better understand the power of RNA-Seq.
Webinar: Getting Started with Whole Genome Sequencing
In this free 30 min webinar, you'll get a brief introduction to Next Generation Sequencing, learn important considerations for designing a Whole Genome Sequencing experiment, understand the WGS-Seq workflow, analysis, and interpretation, as well as learn about other applications such as Plasmid Sequencing & more.
Webinar: Using NGS for CRISPR Validation, Antibody Screening & Metagenomics
In this free 30 min webinar, you'll learn about how Amplicon-Seq can help with common sequencing challenges, get a brief introduction into Next Generation Sequencing, learn important considerations and understand the workflow for amplicon-seq, as well as get an idea of other applications for amplicon sequencing such as CRISPR validation, metagenomics, and antibody screening.
Webinar: NGS Data Analysis 101 for RNA-Seq, WGS, and more
In this free 30 min webinar, you'll learn about how Amplicon-Seq can help with common sequencing challenges, get a brief introduction into Next Generation Sequencing, learn important considerations and understand the workflow for amplicon-seq, as well as get an idea of other applications for amplicon sequencing such as CRISPR validation, metagenomics, and antibody screening.
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